Journal:

Article Title: Molecular Characterization of Benzimidazole Resistance in Helicobacter pylori

doi: 10.1128/AAC.48.7.2524-2530.2004

Figure Lengend Snippet: Strains and vectors

Article Snippet: H. pylori cultures were typically incubated for 48 h after routine subculture and for 3 to 5 days after plating for selection of transformants. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or vector (clone) Relevant genotype, phenotype, or sequence Source or reference H. pylori strains AH244 Wild type 1 J99 Wild type 2 J99A.1 Compound A-resistant J99; NuoD G398S This study J99A.2 Compound A-resistant J99; NuoD F404S This study J99A.3 Compound A-resistant J99; NuoD V407M This study J99A.4 Compound A-resistant J99; NuoB T27A This study E. coli strains ER1793 e14( mcrA6 ) Δ( mcrC-hsdRMS-mrr ) 114::IS 10 27 ; New England BioLabs TOP10 F − , mcrA Δ( mrr-hsdRMS, mcrBC ) φ80 lacZ ΔM15 endA1 recA1 20 ; Invitrogen ECO523 K-12 derivative; F − mcrA mcrB IN( rrnD-rrnE )1 lambda − ATCC 27325 (W3110) ECO524 ECO523 with Tn 10 insertion in tolC Vectors (clones) LambdaGEM-11 Lambda-based cloning vector Promega Clone 17 Lambda GEM-11 containing 17,083-bp fragment from J99 genomic library containing compound A resistance gene This study pUC19 Cloning vector, Ap r 36 pGEM-T Cloning vector, Ap r Promega pILL600 pBR322 containing C. coli aphA -3 gene, Km r 23 pSM126 pUC19 containing 5,707-bp XbaI-SacI fragment from clone 17, Ap r This study pSM127 pUC19 containing 4,032-bp SacI-XbaI fragment from clone 17, Ap r This study pSM128 pUC19 containing 1,908-bp SacI fragment from clone 17, Ap r This study pSM129 pUC19 containing 5,465-bp SacI fragment from clone 17, Ap r This study pSM130 pUC19 containing 3,017-bp SacI-EcoRI fragment from pSM129, Ap r This study pSM131 pUC19 containing 807-bp EcoRI fragment from pSM129, Ap r This study pSM132 pUC19 containing 1,641-bp EcoRI-SacI fragment from pSM129, Ap r This study pSM135 pSM132 containing 718-bp deletion in nuoD replaced by aphA -3 in the same transcriptional orientation This study Open in a separate window Strains and vectors Escherichia coli strains were grown aerobically at 37°C on Luria-Bertani (LB) agar or in broth supplemented with either ampicillin at 100 μg/ml or kanamycin at 50 μg/ml.

Techniques: Plasmid Preparation, Sequencing, Clone Assay

Journal:

Article Title: Involvement of Quinolinate Phosphoribosyl Transferase in Promotion of Potato Growth by a Burkholderia Strain

doi: 10.1128/AEM.72.1.760-768.2006

Figure Lengend Snippet: Physical relationship of clones containing PsJN plant growth-promoting activity and schematic map of genes identified by sequence analysis. PsJN λ6 is an approximately 19-kb Sau3AI fragment in lambda GEM-11. pKK114 contains a 7.2-kb SstI fragment from PsJN λ6 cloned into pRK415. The arrows indicate the translation orientations of the genes. Restriction sites: B, BamHI; S, SstI; X, XhoI.

Article Snippet: The genomic DNA libraries of wild-type strain PsJN and mutant H41, which showed a loss of growth-promoting activity, were constructed with the vector lambda GEM-11 (Promega, Madison, WI).

Techniques: Clone Assay, Activity Assay, Sequencing